Figure 1. Expression of MIR4435 is up-regulated in GC tissues and cell lines. (A) Volcano plot of lncRNAs illustrating the difference in lncRNA expression between GC tissues and paired normal gastric tissues. The red and green symbols depict significantly up- and down-regulated lncRNAs in GC (P < 0.05). (B) Heat map and hierarchical clustering analysis showing the different lncRNA expression profiles in GC and normal samples. The relative expression from high to low level is shown with red and green color. (C, D) Validation of the differential expression of 8 lncRNAs in Zhong cancer tissue and paired normal gastric tissue using RT-qPCR (*P < 0.05, **P < 0.01). (E) Detection of MIR4435-2HG expression in 57 GC samples and 57 adjacent normal gastric samples using RT-qPCR. MIR4435-2HG levels were normalized to GAPDH and expressed in terms of the threshold cycle (CT) ratio (**P < 0.01). (F) Basal expression of MIR4435-2HG in GES-1, HGC-27, SNU-5, and SGC-7901 cells.