Figure 2. Depletion of MIR4435-2HG in GC cells inhibits cell growth and metastasis in vitro. (A) MIR4435-2HG knockdown inhibited cell proliferation (CCK8 assays) (*P < 0.05, **P < 0.01). (B) Colony formation assays were used to evaluate SNU5 and HGC-27 cell proliferation after inhibiting expression of MIR4435-2HG (*P < 0.05, **P < 0.01). (C) The cell cycle was analyzed using flow cytometry after knocking down MIR4435-2HG (**P < 0.01). (D) Flow cytometric apoptosis assays were used to analyze the incidence of apoptosis after silencing MIR4435-2HG (*P < 0.05). (E) MIR4435-2HG inhibition decreased cell migration in transwell assays (*P < 0.05). (F) Transwell assays used to assess the invasiveness of cells with downregulated MIR4435-2HG (**P < 0.01). (G) Expression of N-cadherin, MMP-9, VEGF and α-SMA in SNU5 and HGC-27 cells was detected by WB after decreasing MIR4435-2HG expression.