Figure 3. MIR4435-2HG promotes gastric cancer cell tumorigenesis in vivo. (A) MIR4435-2HG knockdown inhibited subcutaneous tumor formation in nude mouse models. Compared to the HGC-27/shNC group, the HGC-27/shMIR4435-2HG group showed smaller tumor sizes (n=5). (B) Quantitative comparison was made between the shNC and shMIR4435-2HG groups at weekly intervals using t-tests. Data are means ± SD for five samples (**P < 0.01). (C) Individual tumor weights from mice in the two groups (**P < 0.01). (D) Expression of N-Cadherin, MMP-9, VEGF and α-SMA in the two groups was detected using IHC. (E) Expression of MMP-9, VEGF and α-SMA in two groups were detected by WB and quantitated (**P < 0.01).