Figure 1. MiR-181a expression declines in APP/PS1 mice during AD development. (A) The expression of miR-181a in the brain of wild-type (WT) and APP/PS1 mice with increasing indicated age was determined by qRT-PCR analysis. U6 was used as an internal control. Data are expressed as relative to that of 3-month-old mice (n = 8 mice per group). (B–C) The level of Aβ 40 (B) and Aβ 42 (C) in WT and APP/PS1 mice with increasing indicated age was measured by ELISA assay. Data are expressed as ng Aβ 40 or Aβ 42 per mg total protein samples (n = 8 mice per group). All data are mean ± SD, and compared by one-way ANOVA followed by Tukey’s post-hoc tests. **, P < 0.01; NS, not significant.