Research Paper Volume 11, Issue 16 pp 6120—6133

MicroRNA-181a protects against pericyte apoptosis via directly targeting FOXO1: implication for ameliorated cognitive deficits in APP/PS1 mice

Figure 2. Lentiviral overexpression of miR-181a via intrahippocampal injection ameliorates cognitive deficits in APP/PS1 mice. (AE) Lentiviral empty vector or lentiviral miR-181a expressing vector was injected into the hippocampus of APP/PS1 mice aged 5-month-old or 8-month-old. Eight mice were included in each group. One month later, mice were used for subsequent biochemical and behavior analyses. (A) The expression of miR-181a in the brain was determined by qRT-PCR analysis. U6 was used as an internal control. Data are expressed as relative to that of 6-month-old mice infected with lentiviral empty vector. (B) Spatial learning of APP/PS1 mice aged 6-month-old or 9-month-old was measured as escape latency (s) in water maze at different days after training. (CD) Spatial memory of APP/PS1 mice was evaluated by probe trials performed at 24 h following the last training session. The number of platform location crossings (C) and time (s) spent in target quadrant (D) was recorded. (E) Swimming speed of APP/PS1 mice aged 6-month-old or 9-month-old was recorded. All data are mean ± SD, and compared by one-way ANOVA followed by Tukey’s post-hoc tests. **, P < 0.01; *, P < 0.05; NS, not significant.