Research Paper Volume 11, Issue 16 pp 6120—6133

MicroRNA-181a protects against pericyte apoptosis via directly targeting FOXO1: implication for ameliorated cognitive deficits in APP/PS1 mice


Figure 3. MiR-181a ameliorates amyloid plaque deposition in APP/PS1 mice. (AE) Lentiviral empty vector or lentiviral miR-181a expressing vector was injected into the hippocampus of APP/PS1 mice aged 5-month-old or 8-month-old. Eight mice were included in each group. One month later, mice were used for subsequent biochemical analyses. (AB) The level of Aβ 40 (A) and Aβ 42 (B) in APP/PS1 mice was measured by ELISA assay. Data are expressed as ng Aβ 40 or Aβ 42 per mg total protein samples. (CE) The slices of mouse brain were stained with thioflavin-S to show plaques in hippocampus (upper) and cortex (lower). (C) The representative images are shown. The plaques were shown with red fluorescence and cell nuclei were highlighted by DAPI staining. Scale bar, 50 μm. (DE) Quantification analysis of the number (D) and area (E) of amyloid plaque shown as in (C). All data are mean ± SD, and compared by one-way ANOVA followed by Tukey’s post-hoc tests. **, P < 0.01.