Research Paper Volume 11, Issue 16 pp 5895—5923

DNA methylation-based estimator of telomere length

Figure 7. Application of DNAmTL on hTERT-transduced primary human fibroblasts. Panel (A) DNAmTL of primary neonatal fibroblasts without (Donor A and B) or with hTERT (Donor A hTERT and Donor B hTERT) transduction demonstrated linear and continued decrease in value regardless of hTERT status. This contrasts with average telomere length measurement by TRF southern blotting (Panel B), which revealed substantial increase in telomere length of primary neonatal fibroblasts that were transduced with hTERT-expression vector.