Research Paper Volume 11, Issue 16 pp 6199—6216

Attenuated BK channel function promotes overactive bladder in a rat model of obesity

Figure 8. The inhibitory effects of NS1619 on spontaneous phasic contractions are attenuated in DSM strips from HFD rats. Representative recordings of DSM strips isolated from ND (A) and HFD (B) rats, illustrating the concentration-dependent inhibitory effects of NS1619 (0.3-30 μM) on spontaneous phasic contractions. Cumulative concentration-response curves illustrate the effects of NS1619 on the amplitude (C), muscle integral force (D), duration (E), frequency (F) and tone (G) of spontaneous phasic contractions in ND and HFD DSM strips. (H) Summary data illustrating that the inhibitory effects of 30 μM NS1619 on spontaneous phasic contractions in isolated DSM strips were lower in HFD rats than in ND rats. Data are expressed as the mean ± SEM; n = 11, N = 8 in the ND group, n = 11, N = 7 in the HFD group; * P < 0.05 for ND vs. HFD; # P < 0.05 for control vs. NS1619. ND: normal diet; HFD: high-fat diet; NS: not significant; Amp: amplitude; Dur: duration; Freq: frequency.