Research Paper Volume 11, Issue 16 pp 6273—6285

Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma


Figure 1. Elevated kindlin-2 promotes melanoma cell invasion and migration. (A) The mRNA and protein levels of kindlin-2 in six melanoma cell lines and HaCaT, a normal skin cell line. (B) The efficiencies of kindlin-2 knockdown and overexpression were examined by western blotting and qRT-PCR. (C and D) The migration ability of SK28-shNC and SK28-sh-kindlin-2 (C), A375-Vector and A375-kindlin-2 (D) cells was measured by wound-healing assays. (E) Invasion ability was measured in the indicated cells by Matrigel invasion assays. (F) The effects of kindlin-2 knockdown and overexpression on proliferation ability were measured by CCK-8 assays. *p<0.05, **p<0.01, ***p<0.001,****p<0.0001.