Research Paper Volume 11, Issue 16 pp 6273—6285

Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma

Figure 3. High levels of kindlin-2 promote angiogenesis and VEGFA secretion in melanoma cells. (A) The tube formation assays were performed with the conditioned medium of SK28-shNC, SK28-sh-kindlin-2, A375-Vector and A375-kindlin-2 cells. (B) The correlation analysis between the expression of kindlin-2 and VEGFA was performed with the TCGA database. (C) The mRNA and protein levels of VEGFA in the indicated cells wer analyzed by qRT-PCR and western blotting. (D) The levels of VEGFA secreted by the indicated cells were analysed by ELISA. *p<0.05, **p<0.01, ***p<0.001.