Research Paper Volume 11, Issue 16 pp 6358—6370

Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation

Figure 3. Isolation and identification of microparticles from endothelial cells. CD144 was used to mark endothelial cell-derived microparticles (EMP). (A) The positive rate of CD144 in the plasma before isolation; (B) The positive rate of CD144 in the plasma after isolation; (C) The isolated microparticles were further selected, using FCM, with the standards of 100 nm and 1000 nm; (D) Transmission electron microscopy was used to observe the obtained microparticles with a diameter >100 nm.