Figure 5. Cav1.3 knock down aggravated the loss of hair cells after senescence induction and resulted in hearing impairment. (A) immunofluorescence of CaV1.3 (green) and mCherry (red) in the organ of Corti (left) and spiral ganglion (right) of control and CaV1.3 knock down AAV group, nuclei was visualized by DAPI (magnification, ×400, scale bar: 50μm), the right panels are the quantitative analysis of CaV1.3 expression in organ of corti (top) and spiral ganglion (bottom). (B) auditory brainstem response (ABR) (top) and the whole cochlear basilar membrane after DAPI staining (bottom) of NC, CaV1.3 knock down, NC+D-Gal and CaV1.3 knock down+D-Gal group (n=6). (C) phalloidine staining for control and negative control AAV or Cav1.3 knock down AAV infected OC segment explants with H2O2 treatment (n=3), the right panel is the quantitative analysis. Error bars represent mean ± s.d.; *P<0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s. not significant; by one-way analysis of variance (ANOVA) (B, C).