Research Paper Volume 11, Issue 16 pp 6535—6554

Mitochondrial bioenergetic changes during development as an indicator of C. elegans health-span

Figure 1. Lifespan is extended upon mild suppression of different mitochondrial proteins. Kaplan-Meier survival curves of wild-type animals fed bacteria transformed with empty vector pL4440 (con) or with pL4440 vector expressing dsRNA targeting the indicated mitochondrial proteins. Mild suppression of the mitochondrial proteins is achieved in the parental generation of animals using undiluted (a-i) or diluted (j-m) RNAi. % mean lifespan increase compared to control is shown next to the corresponding curve. An average of 60 animals per condition was used in each replica and survival curves of pooled population of animals coming from two to four independent replicas are shown. Refer to Supplementary Table SI for a complete statistical analysis of the lifespan assays.