Research Paper Volume 11, Issue 17 pp 7274—7293

The activation of cardiac dSir2-related pathways mediates physical exercise resistance to heart aging in old Drosophila

Figure 6. Effect of cardiac dSir2 differential-expression on the climbing index and average lifespan in Drosophila. (A) The climbing index change curves with aging of cardiac dSir2 knockdown flies. (B) The climbing index of cardiac dSir2 knockdown flies. The sample size was about 100 flies for each group. (C) The climbing index change curves with aging in cardiac dSir2 overexpression flies. (B) The climbing index of cardiac dSir2 overexpression flies. The sample size was about 100 flies for each group. (E) Fly population survival (%) curve of cardiac dSir2 knockdown flies. The leftmost curve represents the cardiac dSir2 knockdown group, of which flies had the shortest lifespan. (F) The average lifespan of cardiac dSir2 knockdown flies. The sample size was 200–220 flies for each group. (G) Fly population survival (%) curve of cardiac dSir2 overexpression flies. The leftmost curve represents the cardiac dSir2 overexpression group, of which flies had the longest lifespan. (H) The average lifespan of cardiac dSir2 overexpression flies. The sample size was 200–220 flies for each group. P-values for lifespan curves were calculated by the log-rank test. Data are represented as means ± SEM. *P<0.05; **P <0.01.