Research Paper Volume 11, Issue 18 pp 7492—7509

PTEN loss regulates alveolar epithelial cell senescence in pulmonary fibrosis depending on Akt activation

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Figure 2. Loss of PTEN and activation of the AKT pathway in lung tissue from IPF patients. (A) Representative results of IHC staining for PTEN and p-AKT 473 in lung tissues (original magnification, 200× for PTEN and 100× for p-AKT 473). (B) Immunofluorescence staining for both SP-C (red) and PTEN (green) were conducted to examine the spatial distribution of PTEN (original magnification, 200×). (CE) Western blot analysis was applied to detect the expression of PTEN and activation of the AKT pathway. Each dot represents an individual lung tissue. **p < 0.01. Unpaired, two-tailed Student’s t test.