Research Paper Volume 11, Issue 18 pp 7492—7509

PTEN loss regulates alveolar epithelial cell senescence in pulmonary fibrosis depending on Akt activation

Figure 2. Loss of PTEN and activation of the AKT pathway in lung tissue from IPF patients. (A) Representative results of IHC staining for PTEN and p-AKT 473 in lung tissues (original magnification, 200× for PTEN and 100× for p-AKT 473). (B) Immunofluorescence staining for both SP-C (red) and PTEN (green) were conducted to examine the spatial distribution of PTEN (original magnification, 200×). (CE) Western blot analysis was applied to detect the expression of PTEN and activation of the AKT pathway. Each dot represents an individual lung tissue. **p < 0.01. Unpaired, two-tailed Student’s t test.