Figure 3. Decrease of PTEN and activation of the AKT pathway in the senescent cell model. Gradually increasing concentrations of bleomycin was added to culture medium to stimulate A549 cells for 72 hours followed by transfer to fresh FBS-free medium for another 24 hours. (A, B) SA-β-Gal staining was performed to detect cellular senescence (original magnification, 200×). (C, D) The expression of the aging-related marker, P21WAF1, was detected by western blot analysis. (E–G) PTEN loss and AKT pathway activation were observed. Data are shown as the mean ± SEM, n ≥ 3 per group. *p < 0.05, ***p < 0.001. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.