Research Paper Volume 11, Issue 18 pp 7492—7509

PTEN loss regulates alveolar epithelial cell senescence in pulmonary fibrosis depending on Akt activation

Figure 5. Knockdown AKT2, but not AKT1, rescues cell from bleomycin-induced cell senescence. The expression of AKT2 or AKT1 was knocked down by lentiviral vector in A549 cells followed by bleomycin (10 μg/ml) stimulation for 72 hours and transfer to fresh medium for 24 hours. (A, B) Cellular senescence was detected by SA-β-Gal staining (original magnification, 200×). (CE) Western blotting was performed to confirm the change of P21WAF1, PTEN and AKT2. (FG) Cellular senescence after AKT1 knockdown was analyzed by SA-β-Gal staining (original magnification, 200×). (H, I). Expression of P21WAF1 and AKT1 was confirmed by western blotting. Data are shown as the mean ± SEM, n ≥ 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.