Figure 5. Knockdown AKT2, but not AKT1, rescues cell from bleomycin-induced cell senescence. The expression of AKT2 or AKT1 was knocked down by lentiviral vector in A549 cells followed by bleomycin (10 μg/ml) stimulation for 72 hours and transfer to fresh medium for 24 hours. (A, B) Cellular senescence was detected by SA-β-Gal staining (original magnification, 200×). (C–E) Western blotting was performed to confirm the change of P21WAF1, PTEN and AKT2. (F–G) Cellular senescence after AKT1 knockdown was analyzed by SA-β-Gal staining (original magnification, 200×). (H, I). Expression of P21WAF1 and AKT1 was confirmed by western blotting. Data are shown as the mean ± SEM, n ≥ 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.