Figure 6. Inhibition of Akt activation attenuates AEC senescence. Inhibitor (20 μm/L LY294002 or 5 μg/ml MK2206) of the AKT pathway was added 1 hour before bleomycin (10 μg/ml). After 72 hours, the medium was changed to fresh medium for 24 hours. AKT inactivation and P21WAF1 expression induced by LY294002 (A–C) or MK2206 (F–H) were detected by western blotting. Cellular senescence after inhibitor LY294002 (D, E) or MK2206 (I, J) pretreatment was analyzed by SA-β-Gal staining. Data are shown as the mean ± SEM, n ≥ 3 per group. *p < 0.05. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.