Research Paper Volume 11, Issue 19 pp 8103—8119

Disulfiram suppressed ethanol promoted RANKL-induced osteoclastogenesis in vitro and ethanol-induced osteoporosis in vivo via ALDH1A1-NFATc1 axis

Figure 4. Disulfiram rescues alcohol-induced bone loss and reduction in bone mechanical strength in mice. (A) Schematic representation of the animal model. (B) Body weights of mice. (C) Mechanical properties (ultimate stiffness, maximum load, elastic load, and maximum displacement) of femurs. (D) Representative 3D micro-CT reconstruction images of mice in the control, ethanol, and disulfiram groups. (E) Quantitative analyses of morphometric parameters of the bone volume to tissue volume (BV/TV, %), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular spacing (Tb.Sp), trabecular connectivity density (Conn.D), and the structure model index (SMI) of the regions of interest (n = 6). Bar graphs are presented as the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the respective controls.