Figure 1. TTN-AS1 is overexpressed in OS tissue samples and cell lines. (A) The expression of TTN-AS1 was analyzed in 47 pairs of OS tissue samples and adjacent normal bone tissues using RT-qPCR. *P < 0.05 vs. the normal bone tissues. (B) RT-qPCR was performed to determine TTN-AS1 expression in four OS cell lines (HOS, SAOS-2, MG-63, and U2OS) and normal osteoblasts (hFOB1.19 cells). *P < 0.05 vs. hFOB1.19 cells. (C) The Kaplan–Meier survival analysis and logrank test were applied to assess the relation between TTN-AS1 levels and the overall survival of patients with OS. The median value of TTN-AS1 expression among the OS tissue samples was chosen as a cutoff. P = 0.026.