Research Paper Volume 11, Issue 18 pp 7678—7693

Long noncoding RNA TTN-AS1 enhances the malignant characteristics of osteosarcoma by acting as a competing endogenous RNA on microRNA-376a thereby upregulating dickkopf-1

Figure 2. The TTN-AS1 knockdown suppresses the proliferation, migration, and invasiveness but promotes the apoptosis of HOS and MG-63 cells. (A) HOS and MG-63 cells were transfected with either si-TTN-AS1 or si-NC. At 48 h post-transfection, the cells were collected and, then, subjected to RT-qPCR analysis for transfection efficiency evaluation. *P < 0.05 vs. the si-NC group. (B) The CCK-8 assay was conducted to assess cellular proliferation after 0, 24, 48, and 72 h of cultivation of si-TTN-AS1-transfected or si-NC-transfected HOS and MG-63 cells. *P < 0.05 vs. group si-NC. (C) Flow cytometry was performed to determine the apoptotic rate of HOS and MG-63 cells after transfection with either si-TTN-AS1 or si-NC. *P < 0.05 vs. the si-NC group. (D, E) The migratory and invasive abilities of TTN-AS1–deficient HOS and MG-63 cells were analyzed in Transwell migration and invasion assays. *P < 0.05 vs. group si-NC.