Research Paper Volume 11, Issue 18 pp 7678—7693

Long noncoding RNA TTN-AS1 enhances the malignant characteristics of osteosarcoma by acting as a competing endogenous RNA on microRNA-376a thereby upregulating dickkopf-1

Figure 6. DKK1 reintroduction attenuates miR-376a overexpression-mediated suppression of HOS and MG-63 cell proliferation, migration, and invasion as well as miR-376a overexpression-mediated promotion of apoptosis in vitro. Agomir-376a was cotransfected with either plasmid pc-DKK1 or the empty pcDNA3.1 vector into HOS and MG-63 cells. (A) Total protein was isolated from the transfected cells and, then, subjected to western blotting for DKK1 protein quantification. *P < 0.05 vs. group agomir-MC. #P < 0.05 vs. group agomir-376a+pcDNA3.1. (BE) The proliferation, apoptosis, migration, and invasiveness of the aforementioned cells were analyzed by the CCK-8 assay, flow cytometry, and Transwell migration and invasion assays, respectively. *P < 0.05 vs. the agomir-MC group. #P < 0.05 vs. group agomir-376a+pcDNA3.1.