Research Paper Volume 11, Issue 20 pp 8810—8824

Increased calcium channel in the lamina propria of aging rat

Figure 5. Alteration of CACNA1s gene and ECM protein with verapamil treatment in hVFFs. After the verapamil treatment for 6 h in hVFFs, the expression of CACNA1S decreases in RT-PCR as dose-dependent manner (A). The synthesis of collagen I alpha 1 and 2 and collagen III in hVFFs decrease according to the concentration of verapamil (B). However, there are no effect on the synthesis of HA or elastin (C and D). MMP-1 and -8 are increased the expression and activity as the concentration increased (E and F). However, there was no change in the expression of MMP-2 and -9 (E). Relative gene expression (fold change) was normalized to the respective housekeeping gene (18s RNA) controls. HAS, hyaluronic acid synthase. MMPs, matrix metalloproteinases. One-way ANOVA test; NS as no significant, *p<0.05, **p<0.01 and ***p<0.001.