Research Paper Volume 11, Issue 19 pp 8139—8155

Hypermethylated long noncoding RNA MEG3 promotes the progression of gastric cancer

Figure 3. Verification of the target relationship between the three target genes. (A) The RNA pull-down test showed a low level of MEG3 enrichment in the bio-NC group than in the bio-miR-181a-5p, DMSO + 5-Aza and 5-Aza treatment groups. **P<0.01, compared with the bio-NC group. (B) A negative correlation between the expression of MEG3 and miR-181a-5p was detected by linear regression analysis after qRT-PCR detection of 30 tissue specimens. (C) Luciferase reporter assay showed lower luciferase activity in the mimic-miR + lncRNA MEG3-wt group than in the mimic-miR + lncRNA MEG3-mut group. *P<0.05, **P<0.01, compared with the NC group. (D) The expression of ATP4B was verified by qRT-PCR after the overexpression of miR-181a-5p. **P<0.01, compared with the NC group. (E) The RNA pull-down test showed a higher level of ATP4B enrichment in the inhibitor-miR group than in the mimic-miR group. *P<0.05, compared with the NC group. (F) qRT-PCR detection showed a negative relationship between the expression of ATP4B and miR-181a-5p. (G) The expression of miR-181a-5p was verified by qRT-PCR after the overexpression of MEG3. *P<0.05, compared with the NC group. (H) The expression level of each treatment group MEG3. *P<0.05, compared with the NC group.