Research Paper Volume 11, Issue 19 pp 8156—8168

Knockdown of CREB3 activates endoplasmic reticulum stress and induces apoptosis in glioblastoma

Figure 6. The downregulation of CREB3 activated ERS pathway proteins in U251MG cells. (A) The levels of the ERS-related proteins ATF6, p-PERK, p-eIF2α and ATF4 were detected in U251MG cells transfected with the NC or CREB3-shRNA2. (BE) The relative levels of ATF6 (B), p-PERK (C), p-eIF2α (D) and ATF4 (E) were quantified and normalized to β-actin, PERK, eIF2α and β-actin levels respectively. **P<0.01 compared with the NC group.