Figure 6. The downregulation of CREB3 activated ERS pathway proteins in U251MG cells. (A) The levels of the ERS-related proteins ATF6, p-PERK, p-eIF2α and ATF4 were detected in U251MG cells transfected with the NC or CREB3-shRNA2. (B–E) The relative levels of ATF6 (B), p-PERK (C), p-eIF2α (D) and ATF4 (E) were quantified and normalized to β-actin, PERK, eIF2α and β-actin levels respectively. **P<0.01 compared with the NC group.