Research Paper Volume 11, Issue 19 pp 8156—8168

Knockdown of CREB3 activates endoplasmic reticulum stress and induces apoptosis in glioblastoma

Figure 8. The downregulation of CREB3 inhibited U251MG xenograft tumor growth in vivo. (A) Mice were subcutaneously implanted with CREB3-shRNA2-infected U251MG cells, and the xenograft tumor volumes were measured weekly. (B) Tumors were excised from the xenografts and photographed after five weeks. (C) The weights of the tumors were calculated. (D) The CREB3 levels in tumor tissues were detected by qRT-PCR. (E, F) TUNEL staining was performed on tumor tissues from each group, and the percentage of TUNEL-positive cells was calculated. (G) The levels of the Bax and active caspase 3 in tumor tissues were detected by Western blotting. (H) The relative levels of Bax and active caspase 3 were quantified and normalized to β-actin levels. **P<0.01 compared with the NC group.