Research Paper Volume 11, Issue 19 pp 8183—8203

CircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression

Figure 6. Upregulation of circRNA Cdr1as in tumors promoted HCC growth in vivo. (A) Representative images of xenografts tumor (6 mice per group) in nude mice. (B) Xenograft tumor weight. (C) Upregulation of circRNA Cdr1as promotes tumor metastasis in vivo (scale bar: 100μm). (Top) Representative bright field images of lungs. (Bottom) Hematoxylin and eosin (HȦE) staining of lung serial sections. (D) The number of metastatic nodules. (E) Immunohistochemical staining of AFP, Ki-67 and PCNA expression in xenograft tumors (scale bar: 20μm). (F) MiR-1270 co-localized with circRNA Cdr1as in xenografts tumor from NC or circRNA Cdr1as overexpressing group was detected by FISH (scale bar: 20μm). Results were presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001. All of the experiments were performed in triplicate.