Research Paper Volume 11, Issue 19 pp 8183—8203

CircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression

Figure 7. Exosomal circRNA Cdr1as serve as a mediator in intercellular communication. Exosomes (Exos) isolated from the medium of 293T, HepG2 and SMMC-7721 cells. (A) Micrographs of exosomes isolated from HepG2 (left), SMMC-7721 (middle) and 293T cells (right). (B) Western blot analysis of TSG101 and CD63 in exosomes of cell lines. (C) qRT-PCR detection of the fold change of circRNA Cdr1as between exos of HepG2, SMMC-7721 and 293T and their producer cells. (D) Exos of HepG2 and SMMC-7721 cells were labeled with PKH67; green represents PKH67, and blue represents nuclear DNA staining by DAPI. 293T cells were incubated with exos derived from HepG2 and SMMC-7721 cells for 3 h. (E) EdU assays of cell proliferation ability. (F) Representative images of migration assays of 293T cells. The number of cells were counted. Results were presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001. All of the experiments were performed in triplicate.