Figure 1. Kruppel-like factor (KLF) 4 is downregulated in various tissues harvested from klotho (-/-) mice. (A) Expression of mRNA encoding KLF4 in wild type and klotho (-/-) mouse tissues (as measured by qRT-PCR). (B) Protein expression of KLF4 in tissues harvested from wild-type and klotho (-/-) mice. Total protein samples were collected from the skin, liver, kidney, brain, salivary gland, lung, and spleen. The expression of KLF4 protein was determined by Western blot. Actin was used as an internal control.