Research Paper Volume 11, Issue 19 pp 8254—8269

Soluble klotho regulates the function of salivary glands by activating KLF4 pathways

Figure 3. Effects of soluble klotho on the expression of proteins belonging to the KLF4 pathway in wild-type and klotho (-/-) MEFs. (AD) qRT-PCR analysis of soluble klotho and KLF4. Total RNA samples were prepared from soluble klotho-transfected MEFs, and quantitative RT-PCR analysis was performed using the primers described. (E, F) The expression of proteins related to the KLF4 pathway. Wild-type and klotho (-/-) MEFs were transfected with soluble klotho expression plasmids (pcDNA3-soluble klotho). At 48 h after transfection, Western blot analysis was performed to assess the KLF4, mTOR, p70S6K, p21, AMPK, cyclin D1, cyclin B1, SOD1, and SOD2 levels. The mean ± S.D. of three independent experiments is shown (*p < 0.05, **p < 0.01).