Figure 5. The expression of salivary gland functional proteins in primary salivary gland cells (PSGCs). (A, B) The protein and mRNA expression of KLF4 in KL(+/+) PSGCs and KL(-/-) PSGCs. (C–E) Comparison of functional protein or mRNA (aquaporin 5, EGFBP2, ZO-1, and amylase) expression between KL(+/+) PSGCs and KL(-/-) PSGCs by RT-PCR, qRT-PCR, and immunoblot analyses. (F–H) Effects of soluble klotho on functional protein expression. Cells were transfected with pcDNA3-soluble klotho for 48 h. Comparisons of salivary gland functional proteins by RT-PCR, qRT-PCR, and Western blot. The actin protein was used as an internal control. The results are reported as the mean ± SD (*p < 0.05, **p < 0.01).