Figure 5. Influence of aging or high-fat diet feeding on adiponectin, adipoR1, and adipoR2 expression in lacrimal glands. Adiponectin (A), adipoR1 (B), and adipoR2 (C) mRNA expression levels in lacrimal glands. Upper and lower graphs show results in young and aged mice (N=7-8), and in mice fed a normal diet (ND) or high-fat diet (HFD) for 8 weeks (N=4-5), respectively. (D) Detection of the AdipoR2 protein by Western blotting. Lysates prepared from the lacrimal glands of young and aged mice were immunoblotted with anti-AdipoR2 and anti-β-Actin antibodies. Left and right images show male (N=2) and female mice (N=2), respectively. The bar graph shows integrated signal intensities in AdipoR2 normalized to that of β-Actin (N=4). (E) AdipoR2 expression in the lacrimal glands of young and aged mice as detected by immunofluorescence. Nuclei were stained with DAPI. Bars = 40 μm. Values are presented as means ± SEM. NS, not significant. *p<0.05 and **p<0.01 (an unpaired Student’s t-test).