Figure 2. Identification of stem cell-like properties in breast cancer cell lines. (A) Identification of the purity of tumor stem cell markers sorted from magnetic beads. (B) MDA-MB-231 and HCC1806 tumor spheres were dyed with antibodies for breast cancer stem cell-related marker and DAPI for nuclei. Fluorescence images were captured using an Operetta® High Contents Screening System. (C) A representative image of MDA-MB-231-GFP (green) and DiI-labeled HCC1806 (red) cells that were implanted in zebrafish embryos at 48 hpf. Non-CSC cells remained in the yolk sac, whereas CSC cells were distributed to the tail (lower right part shows the magnification image of cell migration (larvae stage, n = 30 per group). Right panel, analysis of the percentage of fish with cells at the trunk.