Research Paper Volume 11, Issue 19 pp 8347—8361

Gomisin M2 from Baizuan suppresses breast cancer stem cell proliferation in a zebrafish xenograft model

Figure 4. Tumor spheres treated with Gomisin M2 for 48 h. Treated MDA-MB-231 (A) and HCC1806 (C) tumor spheres were stained with cell permeability, MMP, cytochrome c, and DAPI dyes. Tumor spheres treated with 0.1% vehicle DMSO were used as negative controls. Doxorubicin was used as positive control. (B) The quantitative data of panel A (MDA-MB-231) tumor spheres. The flourecence intencity of cell permeability, MMP, cytochrome c and total nuclear are shown. (D) The quantitative data of panel C (HCC1806) tumor spheres. The flourecence intencity of cell permeability, MMP, cytochrome c and total nuclear are shown. ** p < 0.01, *** p < 0.0001, t-test. (E) Western blot analysis of cytosolic cytochrome c release in the cytosol of MDA-MB-231 and HCC1806 cell lines after 48 h of treatment, respectively.