Figure 8. Gomisin M2 downregulates the Wnt/β-Catenin pathway in breast cancer cells. The effects of Gomisin M2 on expression levels of cyclin D1, β-catenin, GSK3-β, p-β-catenin, and p-GSK3β in MDA-MB-231 (A) and HCC1806 (B) cells. Cells were stimulated with increasing doses of Gomisin M2 for 48 h, and the same dose was applied at different time points. The experiment was repeated thrice with similar results.