Research Paper Volume 11, Issue 19 pp 8362—8373

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Figure 1. Mogroside V alleviates the deterioration in oocyte quality during in vitro ageing. After in vitro maturation for 44 h, oocytes that extruded the first polar body were continuously cultured in vitro with or without MV (25, 50 and 100 μM) for 24 h. (A) Views of oocytes after activation. (B) Activation rates of oocytes in the fresh, aged, and aged+MV groups. (C) Representative images of blastocysts from fresh, aged and aged+MV oocytes. (D) Blastocyst formation rates of fresh, aged and aged+MV oocytes. MV, mogroside V; Scale bar = 200 μm. The data are presented as the mean ± SEM of at least three independent experiments. * P < 0.05, *** P < 0.001, **** P < 0.0001.