Figure 2. Effects of mogroside V on the ROS level and SIRT1 expression in oocytes during in vitro ageing. After in vitro maturation for 44 h, oocytes that extruded the first polar body were continuously cultured in vitro with or without MV for 24 h. (A) Representative images of fresh, aged and aged+MV oocytes stained with DCFH-DA. (B) Quantitative analysis of ROS fluorescence intensity in fresh, aged and aged+MV oocytes. (C) The mRNA expression of SIRT1 in fresh, aged and aged+MV oocytes. The data are presented as the mean ± SEM of at least three independent experiments. MV, mogroside V; Scale bar = 100 μm. * P< 0.05, ** P < 0.01, **** P< 0.0001.