Figure 6. Effect of mogroside V on early apoptosis in oocytes during in vitro ageing. After in vitro maturation for 44 h, oocytes that extruded the first polar body were continuously cultured in vitro with or without MV for 24 h. (A) Representative images of fresh, aged and aged+MV oocytes stained with Annexin-V. (B) Quantitative analysis of Annexin-V fluorescence intensity. The data are presented as the mean ± SEM of at least three independent experiments. Mogroside V, MV; Scale bar = 50 μm. *** P < 0.001, **** P < 0.0001.