Figure 7. Inhibition of SIRT1 abolishes the protective effect of mogroside V on oocytes during in vitro ageing. After in vitro maturation for 44 h, oocytes that extruded the first polar body were continuously cultured in vitro with MV or MV plus the SIRT1 inhibitor EX527 for 24 h. (A) Representative images of aged, aged+MV and aged+MV+EX-527 oocytes stained with SIRT1 antibody. (B) Quantitative analysis of SIRT1 fluorescence intensity. (C) Representative images of aged, aged+MV and aged+MV+EX-527 oocytes stained with DCFH-DA. (D) Quantitative analysis of ROS fluorescence intensity. (E) Representative images of spindle morphologies and chromosome alignment in aged, aged+MV and aged+MV+EX-527 oocytes. (F) Quantitative analysis of the abnormal spindle formation rate. (G) Quantitative analysis of the misaligned chromosome rate. The data are presented as the mean ± SEM of at least three independent experiments. Mogroside V, MV; Scale bar, A = 50 μm, C = 200 μm, E = 5 μm. * P < 0.05, ** P <0.01, **** P < 0.0001.