Figure 2. Inhibiting Sch9 activity by rapamycin or myriocin treatment decreased H2S production. (A) Diagram showing how rapamycin and myriocin inhibit Sch9 through two different signaling pathways. (B) H2S production by BY4741 was monitored by using lead acetate strips at 24 or 48 hours after inoculation into YPD medium containing the indicated concentrations of myriocin. (C and D) H2S production by BY4741 or sulfur assimilatory mutants was monitored by using lead acetate strips which were replaced every 24 hours after the indicated concentrations of rapamycin were added into overnight culture of YPD (* p<0.05 compared to control).