Research Paper Volume 11, Issue 19 pp 8418—8432

mTORC1-Sch9 regulates hydrogen sulfide production through the transsulfuration pathway

Figure 3. Decreased H2S production in Δsch9 cells is not due to methionine restriction. (A and B) H2S production was monitored 24 hours after inoculation of BY4741 and BY4742 cells or WT and Δsch9 cells (TB50a background) in the presence of indicated concentration of methionine in the SDC medium by using lead acetate strips. (C) Fluorescence microscopy of eGFP expression in BY4741 cell transformed with either pUG35-eGFP (with MET7 promoter) or pRS316-eGFP (without MET7 promoter). (D) Immunoblot analysis of GFP expression in WT and Δsch9 cells (TB50a background) with actin as loading control. Cells were transformed with either pUG35-eGFP (with MET7 promoter) or pRS316-eGFP (without MET7 promoter). The ratios of GFP to Actin are quantified by ImageJ and indicated below the lower panels.