Research Paper Volume 11, Issue 19 pp 8418—8432

mTORC1-Sch9 regulates hydrogen sulfide production through the transsulfuration pathway

Figure 5. Transsulfuration enzymes are involved in the H2S production regulation by mTORC1-Sch9. (A and B) RT-qPCR analysis of CYS3 and CYS4 mRNA expression in TB50a cells in the presence or absence of Sch9 (A) or Rapamycin (B). (C and D) H2S production monitored by lead acetate strips (C) in WT and Δsch9 BY4741 cells or methylene blue assays (D) in WT and Δsch9 TB50a cells overexpressing CYS3 or CYS4 or with empty vector. (E) H2S production monitored by lead acetate strips in WT BY4741 cells overexpressing CYS3 or CYS4 or with empty vector with or without rapamycin treatment. (* p<0.05; *** p<0.005).