Research Paper Volume 11, Issue 19 pp 8433—8462

Brain pyrimidine nucleotide synthesis and Alzheimer disease

Figure 3. Leflunomide effect on neuroblastoma SH-SY5Y cells. N ≥ 3. (A) Cell proliferation (in million cells). *: p < 0.05 versus untreated cells (control). (B) TUBB3 (green) and TH (red) fluorescence intensity (FI) mean and standard deviation values in neuron-differentiated cells. Dashed line (100 %) represents TUBB3 or TH mean values of undifferentiated cells. C: untreated cells; LEF: leflunomide-treated cells; LEF+U, leflunomide plus uridine (200 μM)-treated cells; *: p ≤ 0.0021, versus undifferentiated cells; #: p ≤ 0.0055, versus untreated neuron-differentiated cells. (C) Oxygen consumption in proliferating SH-SY5Y cells. *: p < 0.05, versus untreated cells.