Research Paper Volume 11, Issue 19 pp 8526—8541

Ninjurin 2 overexpression promotes human colorectal cancer cell growth in vitro and in vivo

Figure 6. NINJ2 silencing or depletion inhibits HT-29 xenograft growth in SCID mice. Stable HT-29 cells (6×106 cells per mouse) with NINJ2 shRNA (“Seq3”) or lenti-CRISPR/Cas9-NINJ2 KO construct (“sgRNA-2”), as well as the parental control HT-29 cells (“Ctrl”), were s.c. inoculated into the flanks of the SCID mice. When each tumor was around 100 mm3 in volume, the recording was started. Tumor volumes (A) and mice body weights (D) were recorded every 6 days for a total of 36 days; Estimated daily tumor growth (in mm3 per day) was calculated (B); At Day-36, each tumor was isolated and weighted individually (C); At Day-6 and Day-12, one tumor of each group was separated. The total six tumors were homogenized anddissolved in the tissue lysis buffer, NINJ2 mRNA and listed proteins were tested by by qPCR assay (E) and Western blotting assay (F). Expression of the listed proteins were quantified, normalizing to the loading control protein (F). For each group, n=10. *P<0.05 vs.“Ctrl” tumors.