Research Paper Volume 11, Issue 19 pp 8526—8541

Ninjurin 2 overexpression promotes human colorectal cancer cell growth in vitro and in vivo

Figure 7. NINJ2 knockout inhibits primary human colon cancer cell growth in vivo. The stable primary human colon cancer cells (“pri-Can-1”) with lenti-CRISPR/Cas9-NINJ2 KO construct (“KO-NINJ2”, with “sgRNA-2”), or the parental control cells (“Ctrl”), were s.c. inoculated into the flanks of the SCID mice (6 × 106 cells per mouse). When each tumor was close 100 mm3 in volume, the recording was started (labeled as “Day-0”). Tumor volumes (A) and mice body weights (B) were recorded every 6 days for a total of 36 days; At recording “Day-6” and “Day-12”, one tumor of each group was separated, tumors were subjected to Western blotting assay of listed proteins (C). NINJ1/2 protein expression and Akt-Erk1/2 phosphorylations were quantified (C). For each group, n=8. *P<0.05 vs.“Ctrl” tumors.