Figure 3. Prob treatment alleviates hippocampal neuronal loss in D-gal-induced aging mice. (A) H&E staining. (B) Nissl staining. (C) Cell apoptosis was detected by the TUNEL assay. The representative sections of hippocampal CA1 region were chosen and used to quantify positive cell numbers by a blinded observer. Data in each experiment represent mean ± SEM from 4-5 independent samples. Statistically significant differences were calculated by one-way ANOVA using the SPSS 20.0 software. *P<0.05 and ** P<0.01, versus D-gal group.