Figure 6. Effect of Prob on the expression and dissociation of Keap1/Nrf2 complex. (A) The dissociation of Keap1/Nrf2 complex was assessed by Co-IP assay. (B) The protein expression of Nrf2 in nucleus was assessed by Western blot assay. Data in each experiment represent mean ± SEM from 4-5 independent samples. Statistically significant differences were calculated by one-way ANOVA using the SPSS 20.0 software. ** P<0.01, versus D-gal group.