Research Paper Volume 11, Issue 19 pp 8604—8622

Tryptophan metabolite 5-methoxytryptophan ameliorates arterial denudation-induced intimal hyperplasia via opposing effects on vascular endothelial and smooth muscle cells

Figure 1. 5-MTP reduces arterial denudation-induced intimal hyperplasia. Mice were subjected to femoral artery denudation injury and treated with PBS or 5-MTP. Vessels were harvested 4 weeks later. (AB) Uninjured vessel cross sections were stained with H&E (A) or Verhoeff’s staining for elastin (B). (CF) Sections from injured arteries from vehicle (CD) or 5-MTP-treated (EF) mice were stained for H&E (C and E) or elastin stain (D and F). Representative sections are shown. Arrowheads indicates internal elastic lamina (IEL) and external elastic lamina (EEL), respectively, while a line with arrowhead at both ends delineates neointima (neo). (G) Quantitative morphometric analysis of intimal and medial area and expressed as intima/media ratio in injured vehicle- (1.73±0.35, n=7) and 5-MTP-treated (0.73±0.17, n=9) mice (*p=0.0076 vs. vehicle).