Research Paper Volume 11, Issue 19 pp 8623—8641

TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1

Figure 1. TRIM59-/- macrophages promote B16-F10 tumor growth. (A) Representative images of B16-F10 tumors from three independent experiments. Wild-type mice or TRIM59-CKO mice were inoculated subcutaneously with B16-F10 cells, and sacrificed 28 days later. n = 5 mice per group. (B) Mean tumor volume in the different experimental groups. (C) Overall survival in WT mice and TRIM59-CKO mice implanted with B16 melanoma allografts. n = 10 mice per group. (D) Phenotypic screening of TAMs. Representative FACS plots from WT and TRIM59-CKO mouse macrophages. (E) Flow cytometry analysis of macrophage subpopulations based on Ly6C and MHCII expression. Data are represented as mean ± SD. *p<0.05, compared to WT.