Research Paper Volume 11, Issue 19 pp 8623—8641

TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1

Figure 5. Knockdown of MMP-9 or Madcam1 inhibits migration, invasion, and EMT in melanoma cells. (A) Expression of MMP-9 and Madcam1 in B16-F0 and B16-F10 cells transfected with MMP-9-shRNA, Madcam1-shRNA or scrambled (control)-shRNA, as assessed by qRT-PCR. Data are represented as mean ± SD. **p<0.01, compared with control cells. (B) Expression of MMP9 and Madcam1 in B16-F0 and B16-F10 cells transfected with MMP-9-shRNA, Madcam1-shRNA, or scrambled (control)-shRNA, as assessed by western blotting. A representative image from three independent experiments is shown. (C) Representative images of cell migration assays conducted on B16-F10 cells transfected with control shRNA, MMP-9-shRNA, or Madcam1-shRNA. Data are represented as mean ± SD. *p<0.05, compared with control cells. (D) Representative images of transwell invasion assays conducted on B16-F10 cells transfected with control shRNA, MMP-9-shRNA, or Madcam1-shRNA. Data are represented as mean ± SD. *p<0.05, compared with control cells. (E) Western blot detection of EMT-associated proteins, including E-cadherin, N-cadherin, and vimentin, in B16-F0 and B16-F10 cells after shRNA-mediated downregulation of MMP-9 or Madcam1.